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Old 12-15-2010, 11:20 AM   #1
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Default PubMed: High-throughput sequencing of complete human mtDNA genomes from the Philippin

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High-throughput sequencing of complete human mtDNA genomes from the Philippines.

Genome Res. 2010 Dec 8;

Authors: Gunnarsdóttir ED, Li M, Bauchet M, Finstermeier K, Stoneking M

Because of the time and cost associated with Sanger sequencing of complete human mtDNA genomes, practically all evolutionary studies have screened samples first to define haplogroups and then either selected a few samples from each haplogroup, or many samples from a particular haplogroup of interest, for complete mtDNA genome sequencing. Such biased sampling precludes many analyses of interest. Here, we used high-throughput sequencing platforms to generate, rapidly and inexpensively, 109 complete mtDNA genome sequences from random samples of individuals from three Filipino groups, including one Negrito group, the Mamanwa. We obtained on average ~55-fold coverage per sequence, with
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