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Old 04-12-2018, 12:59 PM   #1
jlove
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Default FuPa reagent?

Can anyone explain how the FuPa reagent works? In the context of Illumina library preparation, The FuPa reagent is a proprietary reagent that is used to partially digest the primers and phosphorylate the amplicons for adapter ligation. Illumina told me it was proprietary.

Thanks in advance!
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Old 04-16-2018, 12:05 AM   #2
torben
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Since it's proprietary no one outside Thermo really knows, but some good guesses can be found in this thread http://seqanswers.com/forums/showthread.php?t=27039
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Old 04-16-2018, 07:30 AM   #3
r.rosati
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This patent record is a nice read regarding the FuPa reagent:
https://patents.google.com/patent/US8728728B2/en
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