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Old 04-12-2018, 01:59 PM   #1
Location: Boston, MA

Join Date: Apr 2011
Posts: 49
Default FuPa reagent?

Can anyone explain how the FuPa reagent works? In the context of Illumina library preparation, The FuPa reagent is a proprietary reagent that is used to partially digest the primers and phosphorylate the amplicons for adapter ligation. Illumina told me it was proprietary.

Thanks in advance!
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Old 04-16-2018, 01:05 AM   #2
Location: Norway

Join Date: Oct 2012
Posts: 15

Since it's proprietary no one outside Thermo really knows, but some good guesses can be found in this thread
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Old 04-16-2018, 08:30 AM   #3
Location: Brazil

Join Date: Aug 2015
Posts: 69

This patent record is a nice read regarding the FuPa reagent:
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