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Old 05-11-2018, 07:07 AM   #1
pedroassoluto
Junior Member
 
Location: france

Join Date: May 2018
Posts: 1
Question condetri illumina sequences trimming

Hello!
I'm new in bioinformatics and I'm trying to launch the program condetri for the quality trimming of my paired end illumuna sequences. in the manual they say to use this command line:
perl condetri.pl -fastq1=file1 [-fastq2=file2 -prefix=s
-cutfirst=i -cutlast=i -rmN -notrim -hq=i -lq=i -frac=[0,1]
-lfrac=[0,1] -minlen=i -mh=i -ml=i -sc=i -pb=s]

so I have prepared the line like that:
perl condetri.pl -fastq1=try1fw.fastq.gz [-fastq2=try1rv.fastq.gz -prefix=s
-cutfirst=0 -cutlast=0 -rmN=no -notrim=no -hq=25 -lq=10 -frac=[0.8]
-lfrac=[0] -minlen=50 -mh=5 -ml=1 -sc=64 -pb=no

...but doesn't work.

it produce an answere like that:
Can't locate object method "TIEHANDLE" via package "IO::Zlib" at condetri.pl line 323.

what does it means and how can I solve it?

thanks guys and sorry for my ignorance.
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