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Old 12-18-2009, 06:58 PM   #1
Location: Maryland

Join Date: Jul 2008
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Default Splicing Analysis in Partek RNA-Seq workflow

Hi, there,

Does anyone know how Partek calculates Alternative Splcing p-value in their RNA-Seq workflow? It seems that they use an EM algorithm to deduce expression of individual transcripts based on the paper in Nucleic Acids Res. 2006 Jun 6;34(10):3150-60. Differential expression p-value is shown for each transcript, but a single alternative splicing p-value is presented for each gene.

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Old 12-21-2009, 09:06 AM   #2
Location: Boston

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Partek has a white paper explaining how to calculate alternative splicing p-value on the tutorial webpage. Open the software, go to "help"--"online tutorials"--looking for "white papers" tab.
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