Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
sequencing 16s rRNA Nullie RNA Sequencing 10 11-16-2016 07:53 AM
16S rRNA Data Analysis with Interactive Results by World Fusion GJ_WF Vendor Forum 0 11-30-2015 11:55 AM
Tool to identify 16s rRNA fragments in raw data Mithril Metagenomics 3 09-23-2012 02:05 AM
PubMed: Exploring Microbial Diversity and Taxonomy Using SSU rRNA Hypervariable Tag S Newsbot! Literature Watch 0 11-22-2008 05:02 AM

Thread Tools
Old 05-27-2016, 01:01 AM   #1
Junior Member
Location: Dublinish

Join Date: Apr 2016
Posts: 4
Default exploring 16s rRNA data

Hey guys,

I am wondering what the limitations of just having 16s rRNA data are for qiime analyses and how quantitative it could possibly be?

uselessAl is offline   Reply With Quote
Old 05-27-2016, 06:58 AM   #2
Senior Member
Location: California

Join Date: Jul 2014
Posts: 198

First off, 16S amplicon sequencing is not quantitative. Your measurements are relative abundances. Other limitations:
- difficult to resolve taxonomy beyond genera
- differences in community profiling depending on primers used
fanli is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 04:22 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO