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Old 05-27-2016, 01:01 AM   #1
uselessAl
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Location: Dublinish

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Default exploring 16s rRNA data

Hey guys,

I am wondering what the limitations of just having 16s rRNA data are for qiime analyses and how quantitative it could possibly be?

Cheers
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Old 05-27-2016, 06:58 AM   #2
fanli
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First off, 16S amplicon sequencing is not quantitative. Your measurements are relative abundances. Other limitations:
- difficult to resolve taxonomy beyond genera
- differences in community profiling depending on primers used
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