Hi. I have a time series RNASEQ experiment with 2 controls and 3 treated samples for each time point.
sample time condition
A 1 control
B 1 control
C. 1 treated
D. 1 treated
E 1 treated
F 2 control
G 2 control
H 2 treated
I 2 treated
J 2 treated
K 3 control
L 3 control
M 3 treated
N 3 treated
O 3 treated
I want to obtain the Differential gene expression for each of the time points and between time points:
time_1_control_vs_treated, time_2_control_vs_treated, time_3_control_vs_treated.
time1_vs_time2
time2_vs_time3
time1_vs_time3
What should I include in the design formula?
Thank you
sample time condition
A 1 control
B 1 control
C. 1 treated
D. 1 treated
E 1 treated
F 2 control
G 2 control
H 2 treated
I 2 treated
J 2 treated
K 3 control
L 3 control
M 3 treated
N 3 treated
O 3 treated
I want to obtain the Differential gene expression for each of the time points and between time points:
time_1_control_vs_treated, time_2_control_vs_treated, time_3_control_vs_treated.
time1_vs_time2
time2_vs_time3
time1_vs_time3
What should I include in the design formula?
Thank you