Gene ontology post IDing using Blast on Geneious

[michael-steffen]

I have a few thousand sequences which I have annotated from blasting to NCBI. I would like to add GO terms to them. I looked into using Blast2go, however, it seems like I have to re-blast sequences and then I can apply go terms. The whole reason I used geneious blast to ncbi was because Blast2go was so slow. Is there a way to by pass the initial blasting again or some other program which is faster.

Thanks,
Mike

[sgoetz]

Hi Mike.
In which format did you save and with which parameters did you run your blast searches? In Blast2GO you can import Blast results as XML. This is possible with the Blast2GO PRO Plugin for Geneious (get a trial from here: https://www.blast2go.com/b2g-plugins/geneious-plugin) as well as the free Blast2GO Basic. In case you do not have your results in XML you would need to redo them. Just for your information, with the PRO (plugin and normal) version you can use Blast2GO's CloudBlast to run NCBI blast+ searches faster than via the NCBI webservice or locally.
Hope this helps.
Best, Stefan