SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
CNV-seq, to detect Copy Number Variation using next-generation sequencing xiechao Literature Watch 46 10-20-2015 09:30 AM
Bioinformatician High-Throughput Sequencing - Norwegian Sequencing Centre tim Academic/Non-Profit Jobs 0 06-22-2011 08:47 AM
PubMed: The edited transcriptome: novel high throughput approaches to detect nucleoti Newsbot! Literature Watch 0 05-17-2011 02:10 AM
Workshop: Informatics on High Throughput Sequencing strob Events / Conferences 1 04-14-2009 07:21 PM
How to detect real deletion or gaps in sequencing projects watashi Bioinformatics 0 08-01-2008 01:15 PM

Reply
 
Thread Tools
Old 01-14-2010, 09:02 PM   #1
zslee
Member
 
Location: shanghai of china

Join Date: May 2009
Posts: 29
Default how high-through sequencing detect I? as G ?

i am doing rna editing analysis using solexa data,
i wann know which base is a I detected as ? even though it's translated as G in vivo
thanks
zslee is offline   Reply With Quote
Old 01-15-2010, 08:52 AM   #2
elaney_k
Member
 
Location: Dublin, Ireland

Join Date: Mar 2008
Posts: 55
Default

I'm not sure if I understand your question, however for detection of A to I RNA editing you need to sequence both the genomic DNA and RNA. If editing occurs you will only see an "A" in the DNA sequence but at the same genomic position in the cDNA (RNA) you will see both As and Gs.
Have a look at this publication for more info:
"Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing" Science. 2009 May 29;324(5931):1210-3.
elaney_k is offline   Reply With Quote
Old 01-15-2010, 04:04 PM   #3
zslee
Member
 
Location: shanghai of china

Join Date: May 2009
Posts: 29
Default

OK,thanks for you reply and sorry for my english
i understand ~~
zslee is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:35 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO