I am getting good alignment to the genome after illumina chipseq but when i call for peaks there are no significant peaks. and the FDR is like 100% for all, even the IgG control. Can i narrow down to whether the antibody or the conditions for binding are not good based on chipseq data!
I donot have a method to verify the pulldown as there are no targets. any suggestions for this, chip western also doesnt work.
Is it ok to crosslink the nuclei prep as the tissue seems to have issues with penetrance?
please do suggest..thanks
I donot have a method to verify the pulldown as there are no targets. any suggestions for this, chip western also doesnt work.
Is it ok to crosslink the nuclei prep as the tissue seems to have issues with penetrance?
please do suggest..thanks
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