Hi all,
We are going to make an experiment involving D melanogaster samples each of wich include about a 25 % of cells being part of a differentially expressed tissue. We are afraid that changes in genes with low expression levels can be masked due to this low representation of 25 %.
Does anybody know what is the more accurate normalization method?
Thank you in advance
We are going to make an experiment involving D melanogaster samples each of wich include about a 25 % of cells being part of a differentially expressed tissue. We are afraid that changes in genes with low expression levels can be masked due to this low representation of 25 %.
Does anybody know what is the more accurate normalization method?
Thank you in advance
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