What would be the benefit from using 2 x 8bp dual indexes vs say... 1 x 16bp single index for a paired end read?
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One good reason is to identify barcode swapping between samples that can happen during PCR:
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How many adapters or primers would you need for each of those scenarios to identify a given number of samples?Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com
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Originally posted by atcghelix View PostOne good reason is to identify barcode swapping between samples that can happen during PCR:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245947/
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