Hello everyone.
I am looking to do some total RNA sequencing. My samples contain both human and bacterial RNA. I was planning on using Ribo-zero Gold Epidemiology to deplete the rRNA however Illumina just announced that Ribo-Zero Gold Epidemiology is being discontinued.
Does anyone know of an alternative to that product that can be used for depletion of both human and bacterial rRNA? I am open to either single-step products, or products that will do human then bacterial (or vice versa) sequentially. Also open to any homebrews that might be out there.
Thanks!
I am looking to do some total RNA sequencing. My samples contain both human and bacterial RNA. I was planning on using Ribo-zero Gold Epidemiology to deplete the rRNA however Illumina just announced that Ribo-Zero Gold Epidemiology is being discontinued.
Does anyone know of an alternative to that product that can be used for depletion of both human and bacterial rRNA? I am open to either single-step products, or products that will do human then bacterial (or vice versa) sequentially. Also open to any homebrews that might be out there.
Thanks!
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