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Old 02-04-2011, 11:07 AM   #1
Dr. Hybe
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Default What happens try to amp/lin/block/hyb paired end libraries on a single read flowcell?

Are the adapters the same or will this make a difference. Thanks
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Old 02-04-2011, 11:19 AM   #2
hoisinjl
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Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.
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Old 02-08-2011, 08:23 PM   #3
gro-seeker
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Quote:
Originally Posted by hoisinjl View Post
Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.
What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?

It looks to me like the adapter sequences are different, and that it wouldn't work the other way around.

Any thoughts?
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Old 02-09-2011, 05:46 AM   #4
kmcarr
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What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?
No, libraries prepared with single read kits are not compatible with paired end cluster generation kits and flowcells.
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Old 02-09-2011, 10:47 AM   #5
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All right, thanks so much! We'll have to go with single reads then.
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