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Old 01-19-2011, 08:14 PM   #1
fishily
Junior Member
 
Location: Pittsburgh

Join Date: Dec 2010
Posts: 1
Default anyone with expertise on cDNA mate-pair library?

Would like to know, if anyone has made a mate-pair library (preferably for Illumina) using RNA as the starting material. I found a PET protocol (454) for full length cDNA and a mate-pair protocol (Illumina) for 2-5 kb DNA, but don't know, if I can combine the two effectively.
Any suggestions?
Thanks!
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