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Old 01-22-2009, 10:10 PM   #1
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Location: California

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Question High-Throughput DNA Shearing


Does anybody have any experience or recommendation about DNA shearing tools that can handle multiple samples simultaneously? I know there are some systems out there that can sonicate a whole plate. Has anybody tried them? Also, I have seen several posts on the Bioruptor: does anybody know if it can go down to 150-200bp peak size? How long does it take?

Thank you so much!

Last edited by ABSOLiDuser; 03-09-2009 at 07:35 PM.
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Old 03-04-2009, 07:20 AM   #2
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We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?
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Old 03-05-2009, 09:50 PM   #3
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Thanks EPI.

Last edited by ABSOLiDuser; 03-09-2009 at 07:34 PM.
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Old 03-08-2009, 05:28 PM   #4
Location: Sydney, Australia

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Default Piranha


I believe ABI is working on a 96 well sonicator for SOLiD called a 'Piranha'. I heard something about it when I attended a SOLiD V3 launch seminar. Talk to your rep and see if this is the case.
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bioruptor, covaris, dna shearing, ht dna shearing, sonication

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