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Old 11-15-2011, 12:56 AM   #1
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Location: Austria

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Default SOLiD library on Ion Torrent

does anyone of you already has experience with putting a SOLiD (small RNA) library on the Ion Torrent?
Which kit or protocol did you use or do you recommend?

Thank you in advance!
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Old 11-16-2011, 12:17 PM   #2
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Hi GenomicIBK,

You can't actually run a SOLiD library on the PGM since the adaptor sequences on the beads are different for each platform. You'll have to make a new Ion library from your RNA.

You can use the Ambion Ion Total RNA-Seq Kit to make your Ion small RNA library. (The protocol is very similar to Ambion's SOLiD Total RNA-Seq kit.) This kit will allow you to make a library that is compatible with the Ion PGM bead prep.
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Old 11-17-2011, 04:32 AM   #3
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It seems I was a bit unclear with my explanation, sorry. My question was, if I already have a SOLiD library, how can I sequence it on Ion Torrent (e.g. as quality control for a multiplexed SOLiD library).
A colleague gave me the tip to simply ligate the primers for Ion Torrent onto my SOLiD library, what I will do now.
I´ll let you know when I have a result.
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Old 11-17-2011, 04:39 AM   #4
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Seems like if you were planning to do this a lot you would want to create Ion Torrent - SOLiD "conversion primers". That way you could make your normal SOLiD library, amplify it with your conversion primers and you would have a Ion Torrent library.

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