Hi,
I am using exome sequencing data to call variants in patient samples and would like to know what quality control is generally applied before mapping. At the moment I am using fastx_quality_filter to filter out reads with <80% at <q30. Is this too stringent?
Thanks
Kath
I am using exome sequencing data to call variants in patient samples and would like to know what quality control is generally applied before mapping. At the moment I am using fastx_quality_filter to filter out reads with <80% at <q30. Is this too stringent?
Thanks
Kath
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