Hi,
I'm new at sequencing and am using the Roche cDNA rapid library prep for 454 Junior sequencing. I fragmented my mRNA as directed using zinc chloride and ran pre- and post-fragmentation samples on the Bioanalyzer pico chip 600. Just wondering if the fragmentation is complete enough. There are spikes at around 150 and 300 nt but still a broad bump peaking at around 2000 nt. I'm attaching the image file.
I'm new at sequencing and am using the Roche cDNA rapid library prep for 454 Junior sequencing. I fragmented my mRNA as directed using zinc chloride and ran pre- and post-fragmentation samples on the Bioanalyzer pico chip 600. Just wondering if the fragmentation is complete enough. There are spikes at around 150 and 300 nt but still a broad bump peaking at around 2000 nt. I'm attaching the image file.
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