Hi everyone,
My lab is looking into performing whole-exome sequencing on a small sample of human subjects on the NextSeq 500. I am responsible for researching the budget involved in such an endeavor and, as a newbie to this subject, would like to seek your advice on the number/type of sample prep/reagent kits to achieve our sequencing goals.
We would like to whole-exome sequence 4-8 (depending on total costs) human subjects at >100x coverage, pair-ended read using the Nextera Rapid Capture Exome Kit and, ideally, the High Output 300 cycle NextSeq Kit.
So, if we use 1 Nextera Rapid Capture Exome Kit (8rxn x 6plex), which according to documentation at 6plex can sequence 6 exome samples using NextSeq 500 High-Output kit, and 1 NextSeq Kit, High Output (300 cycle), can I whole-exome sequence up to 6 unique human subjects at >100x coverage with pair-ended read?
edit: As a follow up question, I'm assuming that 1 Nextera Rapid Capture Exome Kit (8rxn x 6plex) can support sequencing of up to 48 exome samples, by the 8 reaction x 6 plex criteria? I apologize for my ignorance, thank you very much for your help.
Thank you for your help.
My lab is looking into performing whole-exome sequencing on a small sample of human subjects on the NextSeq 500. I am responsible for researching the budget involved in such an endeavor and, as a newbie to this subject, would like to seek your advice on the number/type of sample prep/reagent kits to achieve our sequencing goals.
We would like to whole-exome sequence 4-8 (depending on total costs) human subjects at >100x coverage, pair-ended read using the Nextera Rapid Capture Exome Kit and, ideally, the High Output 300 cycle NextSeq Kit.
So, if we use 1 Nextera Rapid Capture Exome Kit (8rxn x 6plex), which according to documentation at 6plex can sequence 6 exome samples using NextSeq 500 High-Output kit, and 1 NextSeq Kit, High Output (300 cycle), can I whole-exome sequence up to 6 unique human subjects at >100x coverage with pair-ended read?
edit: As a follow up question, I'm assuming that 1 Nextera Rapid Capture Exome Kit (8rxn x 6plex) can support sequencing of up to 48 exome samples, by the 8 reaction x 6 plex criteria? I apologize for my ignorance, thank you very much for your help.
Thank you for your help.
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