SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
question about stranded RNA seq StephaniePi83 RNA Sequencing 11 09-02-2012 05:25 PM
single stranded DNA ChIP-Seq using Solexa litd Illumina/Solexa 3 05-27-2011 02:42 AM
Single stranded coverage from sam/bam ewilbanks Bioinformatics 2 05-14-2011 12:13 AM
strange result of Double-stranded cDNA Synthesis wayland Illumina/Solexa 1 11-29-2010 09:23 AM
Library prep from single-stranded DNA? genlyai Sample Prep / Library Generation 3 11-12-2010 01:26 PM

Reply
 
Thread Tools
Old 06-22-2012, 04:14 AM   #1
TonyBrooks
Senior Member
 
Location: London

Join Date: Jun 2009
Posts: 298
Default Stranded TruSeq

Has anyone adapted the second strand cDNA step of the Illumina TruSeq RNA protocol to generate stranded libraries by the dUTP method?
Did it work?
TonyBrooks is offline   Reply With Quote
Old 06-23-2012, 09:09 AM   #2
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
Default

Quote:
Originally Posted by TonyBrooks View Post
Has anyone adapted the second strand cDNA step of the Illumina TruSeq RNA protocol to generate stranded libraries by the dUTP method?
Did it work?
Looks like "Treebeard" got it to work. Also posted his protocol (note presumes you use the v1 manual, I think).

--
Phillip
pmiguel is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:29 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO