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Old 10-01-2012, 01:00 PM   #1
seqseq123
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Default How does new Truseq Stranded Protocol actually work? Is it dUTP based??

How does new Truseq Stranded Protocol actually work? Is it dUTP based??
I have not done this work. Please advise. thank you very much.
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Old 10-01-2012, 01:35 PM   #2
pmiguel
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Yes it is dUTP based. Also adds actinomycin D to first strand synthesis, presumably to prevent any second strand synthesis during that step.

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Old 10-01-2012, 03:24 PM   #3
seqseq123
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Philip, Thank you very much clarifying this. Did you use this in your laboratory? and how happy are you with this?
thank you.
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Old 10-02-2012, 04:52 AM   #4
pmiguel
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Have a kit, did not try it yet. Next week, maybe.

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