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  • The Best Robot for NGS Library Prep

    Hi everyone,

    I wanted to get some insight on a robot for NGS library prep. I am a Lab Mgr. of a NGS core that currently runs 2 MiSeqs. So far we have been considering the EpMotion 5075 but it is quite expensive and I wanted to get some insight from some folks that may have experience integrating these types of robots into their workflows. Thanks for your help.

  • #2
    Have you considered the NGS Express?



    We're pretty high throughput so do most of our library prep on Sciclones, but the NGS Express still gets quite a bit of use for lower throughput projects in the lab. Should handle most of your critical MiSeq applications.

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    • #3
      We use Biomek FXs and Hamilton Star and Starlets, but I don't know if that would really solve your cost problem, since they're both pretty large and expensive-looking. Another consideration you might have to take into account is whether or not you need automation personnel for support.
      We're not super high throughput though, about 100 samples a day.

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      • #4
        Is there any liquid handling users that can give some feedback?
        Its for a genomic core facility with a Illumina platform technology (Miseq, NextSeq or HiSeq).

        epMotion 5075 vs Hamilton STAR vs Sciclone NGS.

        -Consumables
        -Optimization

        Thanks in advance

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        • #5
          I work for Hamilton Robotics as the Scientific Leader for NGS, but came from Beckman Coulter where I was the application scientist developing the NGS methods for them. If you would like to PM me and ask specific questions about automating NGS protocols, I can answer them.

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          • #6
            We use Wafergen Apollo.

            They have protocols for all the basic uses (DNA,RNA,ChIP) and are creating new ones all the time.

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