Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Problem with TruSeq Custom Amplicon Low Input Libraries

    Hi all!
    We need some help with TruSeq Custom Amplicon Low Input libraries 
    We have been processing samples (fresh and FFPE) for a while using this Kit and everything worked fine. Before starting the protocol, we always perform the qPCR to check the quality of the samples and we choose the DNA input as described in the userguide.

    Lately, we are experiencing some problems that we don't understand and that seem to be "hazardous", we are not able to see what's going on …the issue is that we are getting very strange libraries's profiles on the Bioanalyzer 2100 (attached images). Some libraries are not working, others are working ok, and some of them are having some strange peaks before the library (they seem like a kind of "horns") that we can't get ride off, despite purifying the samples again and again….Moreover, it seems that these libraries are carrying something that make the bioanalyzer run not properly, dirty or similar…

    Any one has experienced this issue? Any idea of what could it be? The kit is the same that used to work fine some days before, and the controls are working ok randomly!

    Thanks a lot in advance!!

    Click image for larger version

Name:	Presentación1.jpg
Views:	2
Size:	92.0 KB
ID:	309038

  • #2
    Hi Helana,

    Have you cleaned your BioA recently? These sort of profiles can be seen when the pins are dirty.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM
    • seqadmin
      The Impact of AI in Genomic Medicine
      by seqadmin



      Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
      02-26-2024, 02:07 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-14-2024, 06:13 AM
    0 responses
    32 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-08-2024, 08:03 AM
    0 responses
    71 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-07-2024, 08:13 AM
    0 responses
    80 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-06-2024, 09:51 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X