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  • help: time of adapter ligation

    hello everyone , i'am sample prepation of mRNA, and i have a question about adapter ligation to the dsCDNA:
    the protocol of illuminate write: incubate the sample at room temperature for 15 minutes.
    as we know we usually make a ligation action overnight, i wonder if 15 minutes is enough for ligation reaction?
    thanks! hopes your join!

  • #2
    If you use the illumina kit to construct library, 15 min is enough for adaptor ligation; if not, the ligation time should be optimized.
    Last edited by floor121; 08-16-2011, 10:41 AM.

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