NextSeq 1000 and 2000 cluster density and number of copies

Dornfield · 02-20-2020, 02:40 AM

Any knowledge on the cluster dimensions of the new systems, how many copies of the library are present in each cluster and how these flow cells differ from the other patterned arrays that have become the hallmark of the newer Illumina systems?

Thanks for any insight.

Dornfield · 02-27-2020, 02:15 AM

287 views and no responses! Does that mean no one knows, or no one is interested? It is just a academic question really: how small can Illumina go with this?

GenoMax · 02-27-2020, 04:10 AM

Since these systems have not been released as yet it is not surprising that the information is not yet available. Only thing publicly available seems to be this spec sheet.

SNPsaurus · 02-27-2020, 09:45 PM

The Omics Omics blog by Keith Robison is a good source of information about new systems, but he also wonders how many copies are in a cluster. http://omicsomics.blogspot.com/2020/...hes-super.html

kcchan · 02-28-2020, 10:12 AM

Illumina just posted two sample runs on BaseSpace. You should be able to download the data and see for yourself.

https://basespace.illumina.com/datacentral

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02-20-2020, 02:40 AM	#1
Dornfield Member Location: Oxford Join Date: Aug 2010 Posts: 11	NextSeq 1000 and 2000 cluster density and number of copies Any knowledge on the cluster dimensions of the new systems, how many copies of the library are present in each cluster and how these flow cells differ from the other patterned arrays that have become the hallmark of the newer Illumina systems? Thanks for any insight.

02-27-2020, 09:45 PM	#4
SNPsaurus Registered Vendor Location: Eugene, OR Join Date: May 2013 Posts: 523	The Omics Omics blog by Keith Robison is a good source of information about new systems, but he also wonders how many copies are in a cluster. http://omicsomics.blogspot.com/2020/...hes-super.html __________________ Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

02-27-2020, 02:15 AM	#2
Dornfield Member Location: Oxford Join Date: Aug 2010 Posts: 11	287 views and no responses! Does that mean no one knows, or no one is interested? It is just a academic question really: how small can Illumina go with this?

02-27-2020, 04:10 AM	#3
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,143	Since these systems have not been released as yet it is not surprising that the information is not yet available. Only thing publicly available seems to be this spec sheet.

02-28-2020, 10:12 AM	#5
kcchan Senior Member Location: USA Join Date: Jul 2012 Posts: 186	Illumina just posted two sample runs on BaseSpace. You should be able to download the data and see for yourself. https://basespace.illumina.com/datacentral