Hi all!
We used NimblGen's SeqCap EZ Human Exome Library v2.0 followed by paired-end sequencing on the Illumina GAIIx for exome sequencing. BWA was used for sequence alignment. About 95% of the reads could be aligned to the genome. However, only about 60% of all aligned reads align within the target region. NimbleGen says, that 80% on target is feasible.
Did anyone reach 80% with this library? Any experiences with this library are welcome. We used the SeqCap v.1 library first and achieved the same percentage on target (60%). So the new version did not improve the results in our case. Merely the target region is larger now.
Best wishes,
Ulli
We used NimblGen's SeqCap EZ Human Exome Library v2.0 followed by paired-end sequencing on the Illumina GAIIx for exome sequencing. BWA was used for sequence alignment. About 95% of the reads could be aligned to the genome. However, only about 60% of all aligned reads align within the target region. NimbleGen says, that 80% on target is feasible.
Did anyone reach 80% with this library? Any experiences with this library are welcome. We used the SeqCap v.1 library first and achieved the same percentage on target (60%). So the new version did not improve the results in our case. Merely the target region is larger now.
Best wishes,
Ulli
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