Dear All,
I am new to small RNA Seq data.
I have just received data and before I map my samples I would like to remove 5 and 3' adapters.
Here is my FastQC output, is similar in all samples:
There's something wrong?
Data is 50 bp x single read.
What are all these over-represented sequences? The first more than half of all reads!
Any suggestion?
Many Thanks
paolo
I am new to small RNA Seq data.
I have just received data and before I map my samples I would like to remove 5 and 3' adapters.
Here is my FastQC output, is similar in all samples:
Code:
sequence count percentage possible source GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG 4842248 56.89688396826158 No Hit GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG 496150 5.829810654236004 No Hit GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC 250074 2.9383937711325494 No Hit GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG 226967 2.6668842784641402 No Hit GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT 204732 2.4056208704283897 No Hit TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC 82348 0.9675969923511569 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp) TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC 65591 0.7707006159870881 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp) TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 56948 0.6691445271337941 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp) TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC 45012 0.5288953686757453 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
Data is 50 bp x single read.
What are all these over-represented sequences? The first more than half of all reads!
Any suggestion?
Many Thanks
paolo
Comment