Hi Seqanswer team, and forum members,
Pardon me if this seems to be a broad and naive question.
I do not use sequencing kits, neither I do the quality checks of the data that immediately comes out of the sequencer. I deal with the data that comes after all these checks, and thus, I remain oblivious to what goes on behind the scenes before and during sequencing.
I'm seeing PhiX term in 16s rRNA papers, and its concentration.
I found these urls:.
Concentration varies from sequencing platform, and what you're sequencing. For low diversity sample, sometimes ~10% is suggested sometimes ~50%.
My query:
- I'd like a basic (read as superficial) understanding/definition what is the importance of PhiX?
How does an external element helps to have a better quality data?
- Doesn't it contaminate primers, bar code, indices?
Pardon me if this seems to be a broad and naive question.
I do not use sequencing kits, neither I do the quality checks of the data that immediately comes out of the sequencer. I deal with the data that comes after all these checks, and thus, I remain oblivious to what goes on behind the scenes before and during sequencing.
I'm seeing PhiX term in 16s rRNA papers, and its concentration.
I found these urls:.
Concentration varies from sequencing platform, and what you're sequencing. For low diversity sample, sometimes ~10% is suggested sometimes ~50%.
My query:
- I'd like a basic (read as superficial) understanding/definition what is the importance of PhiX?
How does an external element helps to have a better quality data?
- Doesn't it contaminate primers, bar code, indices?
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