Hi all,
I wonder if it is possible to assess some useful cluster metrics (FWHM or %>=Q30) not for the entire loaded pool but for individual barcodes (or maybe even for separate reads)? The Illumina SAV can show such stats for particular lanes/tiles but does not track the barcodes. In principle, each read can be tracked back to its location in the flow cell by coordinates extraced from the read name, but is there a way to relate it to a particular cluster and get its size?
I wonder if it is possible to assess some useful cluster metrics (FWHM or %>=Q30) not for the entire loaded pool but for individual barcodes (or maybe even for separate reads)? The Illumina SAV can show such stats for particular lanes/tiles but does not track the barcodes. In principle, each read can be tracked back to its location in the flow cell by coordinates extraced from the read name, but is there a way to relate it to a particular cluster and get its size?
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