Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • addSolexaReads.perl error

    Hey guys,

    I'm trying to use the command addSolexaReads.perl to add the rest of my fastq reads to an assembly made with Newbler, but I keep getting the error "-ace must be specified." I think I'm including all of the correct files for the addSolexaReads.perl command but still keep getting that error.

    Has anyone come across this before or might know where the problem is coming from?

    Thanks!

  • #2
    Syntax has changed

    The addSolexaReads.perl syntax changed around consed 23.

    Old syntax:
    Code:
    addSolexaReads.perl your_ace_file.ace your_fileoffilenames.fof your_fasta.fasta
    New syntax, where you explicitly specify the options:
    Code:
    addSolexaReads.perl -ace your_ace_file.ace -fastqfof your_fileoffilenames.fof -fasta your_fasta.fasta
    More:
    This video tutorial demonstrates how to align reads generated by Ion Torrent, 454, or Illumina sequencing to a reference sequence using consed. (Note that the…


    --Dan

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    10 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    51 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X