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  • Illumina TruSeq resuspension Buffer

    Hello,
    Does anyone know what the resuspension buffer in the TruSeq RNA library prep kit contains/what the ingredients are? Is it comparable with 10 mM TRIS?

    Thanks,
    Linda

  • #2
    I'm sure it also contains Tween-20. They recommend adding it to prevent the DNA sticking to the tube

    Comment


    • #3
      10 mM Tris-HCl, pH 8.5

      Comment


      • #4
        Is anyone certain about whether or not Tween-20 is involved?

        Comment


        • #5
          We resuspend all our libraries in Low TE with 0.1% Tween-20

          The Tween-20 prevents libraries sticking to the microtubes.

          Comment


          • #6
            Is it ok to submit for sequencing in those resuspension buffer of Truseq RNA library kit?

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            • #7
              Originally posted by linvei91 View Post
              Is it ok to submit for sequencing in those resuspension buffer of Truseq RNA library kit?
              Yes it is.
              Josh Kinman

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