Hi everyone,
I want to sequence two small RNA libraries by Solexa, and don't need very deep coverage. So I want to mix these two libraries in one run. I knew from this literature that we can add indexing nucleotides to the individual cDNA libraries so that the origin of the sequences can be traced. But that literature didn't tell me how to do. Is anyone here has sequenced the mix small RNA libraries by Solexa? I wonder where to add the indexing nucleotides, 3' adaptor or 5' adaptor? And whether should I change the sequence of RT, PCR and sequence primer.
I want to sequence two small RNA libraries by Solexa, and don't need very deep coverage. So I want to mix these two libraries in one run. I knew from this literature that we can add indexing nucleotides to the individual cDNA libraries so that the origin of the sequences can be traced. But that literature didn't tell me how to do. Is anyone here has sequenced the mix small RNA libraries by Solexa? I wonder where to add the indexing nucleotides, 3' adaptor or 5' adaptor? And whether should I change the sequence of RT, PCR and sequence primer.
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