I am trying to identify miRNAs (novel and conserved) in a plant species using small RNA deep sequencing and a draft genome (~8x coverage). The only problem is that we are not able to release the genome publicly yet. I have seen that there are online programs that have published genomes uploaded - miRcat, miRanalyzer, etc.
I would like to run a program on my computer to identify miRNAs based on sRNA SEQ and a draft genome. I know there is miRDeep but this seems to be specifically for animal miRNAs which have a more constrained hairpin structure.
Does anyone know of any programs that I could run on my own computer, not on the internet, to identify miRNAs using my sRNA SEQ and draft genome?
Does anybody have any other ideas? - Thanks
I would like to run a program on my computer to identify miRNAs based on sRNA SEQ and a draft genome. I know there is miRDeep but this seems to be specifically for animal miRNAs which have a more constrained hairpin structure.
Does anyone know of any programs that I could run on my own computer, not on the internet, to identify miRNAs using my sRNA SEQ and draft genome?
Does anybody have any other ideas? - Thanks
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