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  • cummeRbund error

    Hi guys
    for some reason cummerbund in R cannot read my diffout folder.
    this is the command that I used after installing the library of course
    cuff<-readCufflinks()
    I got the following errors
    No records found in Eutrema_STAR_Cuffdiff/tss_groups.fpkm_tracking
    TSS FPKM tracking file was empty.
    Reading Eutrema_STAR_Cuffdiff/tss_group_exp.diff
    No records found in Eutrema_STAR_Cuffdiff/tss_group_exp.diff
    Reading Eutrema_STAR_Cuffdiff/splicing.diff
    No records found in Eutrema_STAR_Cuffdiff/splicing.diff
    Reading Eutrema_STAR_Cuffdiff/tss_groups.count_tracking
    No records found in Eutrema_STAR_Cuffdiff/tss_groups.count_tracking
    Reading read group info in Eutrema_STAR_Cuffdiff/tss_groups.read_group_tracking
    No records found in Eutrema_STAR_Cuffdiff/tss_groups.read_group_tracking
    Reading Eutrema_STAR_Cuffdiff/cds.fpkm_tracking

    and then when I ran the cuff command I got the wrong number of samples and zero TSS, CDS...etc

    Help plz!

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