If I use the MiSeq v3 kits, according to the denature & dilute guide (attached), I dilute both my PhiX and library concentration to the same concentration, and spike in the desired Phix percentage. For example, if I want to load 15pM at 25% PhiX, I denature and dilute both Phix and library to 15pM, then 150uL of 15pM PhiX + 450uL of 15pM library.
If I use MiSeq v2 kits, the guide says to dilute PhiX to 12.5pM. If I wanted to load the same 15pM at 25% PhiX, what is the calculation? Am I having PhiX at 12.5pM and the library at 15pM dilution? How to do this for v2 kits?
If I use MiSeq v2 kits, the guide says to dilute PhiX to 12.5pM. If I wanted to load the same 15pM at 25% PhiX, what is the calculation? Am I having PhiX at 12.5pM and the library at 15pM dilution? How to do this for v2 kits?
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