Hello All,
I have multiple alignments generated to DEG analysis. I know that grep for unaligned reads is not trust and the best way is the -f4 flag. But here what I want is the contigs with no alignment with those reds from specific libraries.
I already have the counts matrices and I can grep those sequences from the matrix, but I want to do a sanity check .
Thank's in advanced.
I have multiple alignments generated to DEG analysis. I know that grep for unaligned reads is not trust and the best way is the -f4 flag. But here what I want is the contigs with no alignment with those reds from specific libraries.
I already have the counts matrices and I can grep those sequences from the matrix, but I want to do a sanity check .
Thank's in advanced.
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