This is a question I have been hearing lately. Malaysia has not own any NGS platform yet but it's coming (I know at least 4) and probably will arrive by end of this year. When I ask which platform, they say they're not sure (or they are not sure if they wanna let me know).
I was told that many of the NGS machines were owned by small labs rather than big genome center. My lab used to have plans to buy a 454 machine too. After doing all the mathematics and economics, we decided not to.
Yes, it's so much cheaper when you run sequencing on your own. And you can do lotsa things like barcoding your samples up to 4000+ with 6nt barcode. (If you have a software that can sort so many different samples).
There are limitations if you use the service provided by others (I should know better)
Just found an article in Genome Technology published in april 08.
"Is Next-Gen Technology Right for Your lab?"
Some of the things you might wanna consider before you purchase a machine:
1) Data
The data storage will be more expensive than generating the data. Another problem is slow data transfer rate.
2) Cost
Extra funding like data storage upgrade, technical support and machine storage environment etc should be taken into consideration.
They found out that Solexa can perform better in a cold environment.
3) Labour (Hire someone with experience)
4) Test run the machine (Optimization could be weeks or months)
5) How long it takes before the machine is being replaced by a new technology?
Usually 5 years. Anything beyond that will be called LGS (last generation sequencing) 3rd Generation Sequencing will be here in 2010.
Then again, please correct me if I'm wrong.
Cheers
I was told that many of the NGS machines were owned by small labs rather than big genome center. My lab used to have plans to buy a 454 machine too. After doing all the mathematics and economics, we decided not to.
Yes, it's so much cheaper when you run sequencing on your own. And you can do lotsa things like barcoding your samples up to 4000+ with 6nt barcode. (If you have a software that can sort so many different samples).
There are limitations if you use the service provided by others (I should know better)
Just found an article in Genome Technology published in april 08.
"Is Next-Gen Technology Right for Your lab?"
Some of the things you might wanna consider before you purchase a machine:
1) Data
The data storage will be more expensive than generating the data. Another problem is slow data transfer rate.
2) Cost
Extra funding like data storage upgrade, technical support and machine storage environment etc should be taken into consideration.
They found out that Solexa can perform better in a cold environment.
3) Labour (Hire someone with experience)
4) Test run the machine (Optimization could be weeks or months)
5) How long it takes before the machine is being replaced by a new technology?
Usually 5 years. Anything beyond that will be called LGS (last generation sequencing) 3rd Generation Sequencing will be here in 2010.
Then again, please correct me if I'm wrong.
Cheers
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