Hi all,
I just got some RNA-seq data and mapped them to hg19 by tophat, the RNA enrichment was based on RiboMinus rRNA depletion,
but the mapping rate is just 41%. How to explain this? What should I do with those unmapped reads next? I am really new in this field, would you please help me? Any reply will be appreciate.
Vivienne
I just got some RNA-seq data and mapped them to hg19 by tophat, the RNA enrichment was based on RiboMinus rRNA depletion,
but the mapping rate is just 41%. How to explain this? What should I do with those unmapped reads next? I am really new in this field, would you please help me? Any reply will be appreciate.
Vivienne
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