Hi all,
We are slated to do some 454Junior sequencing runs for some colleagues. They prepared the library from cDNA mostly following the Roche standard Titanium protocol but they used their own adaptors. We have a 454Junior and Lib-L and Lib-A emPCR kits in house. I suspect there is a compatibility issue but I'm not sure if we have to add Rapid library adaptors to the library to make it compatible wit the 454Junior chemistry or if we have to buy different emPCR and sequencing kits altogether. Anyone run standard Titanium libraries on a Junior? Thoughts?
We are slated to do some 454Junior sequencing runs for some colleagues. They prepared the library from cDNA mostly following the Roche standard Titanium protocol but they used their own adaptors. We have a 454Junior and Lib-L and Lib-A emPCR kits in house. I suspect there is a compatibility issue but I'm not sure if we have to add Rapid library adaptors to the library to make it compatible wit the 454Junior chemistry or if we have to buy different emPCR and sequencing kits altogether. Anyone run standard Titanium libraries on a Junior? Thoughts?
Comment