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  • Samtools how many reads were mapped as a pair

    Hey, I am new to bioinformatics. I get stuck on the question How many reads were mapped as a pair.
    I have created a .bam file that only contains mapped reads. Then, I need to figure out three questions.
    How many reads were mapped?
    How many reads were mapped as a pair?
    How many reads were mapped as a "proper" pair?
    I can solve the 1st and 3rd question, but I am confusing about the 2nd question. After searching on google, I have found the following code:
    samtools view -F 0x40 filename.bam | cut -f1 | sort | uniq | wc -l
    samtools view -f 0x40 -F 0x4 filename.bam | cut -f1 | sort | uniq | wc -l #left mate
    samtools view -f 0x80 -F 0x4 filename.bam | cut -f1 | sort | uniq | wc -l #right mate
    source from :https://gist.github.com/davfre/8596159
    I don't sure it works or not. Can anyone help me?

    Also, I need to find the Arabidopsis thaliana gene that associated to AT3G13276 and identify how many inserts mapped to that region. Does this need to use IGV to solve it?
    Thanks.

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