Hi,
I have a very basic question.
Why is the read highest achievable read-length of MiSeq compared to HiSeq longer although, as far as I understand, they use the same chemistry?
Second question would be, why is it usual that the second read of a paired end read has lower quality than the first one?
I am a novice in the NGS field. I searched the forum but couldn't find an answer.
Are there any comprehensive introduction to illumina sequencing technology?
The manuals on the illumina webpage are nice, but only give a very rough overview.
Thanks a lot.
kind regards
I have a very basic question.
Why is the read highest achievable read-length of MiSeq compared to HiSeq longer although, as far as I understand, they use the same chemistry?
Second question would be, why is it usual that the second read of a paired end read has lower quality than the first one?
I am a novice in the NGS field. I searched the forum but couldn't find an answer.
Are there any comprehensive introduction to illumina sequencing technology?
The manuals on the illumina webpage are nice, but only give a very rough overview.
Thanks a lot.
kind regards
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