Hey,
has anyone had issues with the enrichment step where the white beads literally ALL attract and stick to the brown beads. They congregate in a way where they are not in between the brown beads but instead coat the brown beads from the outside. Their attraction is relatively weak and the white beads break off very easily, however, this leads to quite a few washing steps (40+).
One thing of note is that the library I received was not very clean with both genomic and adapter contamination quite noticeably in a bioanalyzer.
If anyone has had this problem and a solution please give help!
Thank you so much.
has anyone had issues with the enrichment step where the white beads literally ALL attract and stick to the brown beads. They congregate in a way where they are not in between the brown beads but instead coat the brown beads from the outside. Their attraction is relatively weak and the white beads break off very easily, however, this leads to quite a few washing steps (40+).
One thing of note is that the library I received was not very clean with both genomic and adapter contamination quite noticeably in a bioanalyzer.
If anyone has had this problem and a solution please give help!
Thank you so much.
Comment