Go Back   SEQanswers > Sequencing Technologies/Companies > Pacific Biosciences

Similar Threads
Thread Thread Starter Forum Replies Last Post
Ligation of Illumina adapters to custom adapters ale Sample Prep / Library Generation 2 02-29-2016 12:42 AM
Create perl program vineetha Bioinformatics 3 10-01-2015 12:03 PM
help to create heat map dinesh21.ksd Bioinformatics 1 04-09-2015 05:06 AM
HOW to create image like this? zcrself Illumina/Solexa 1 09-21-2010 04:38 AM

Thread Tools
Old 09-12-2018, 02:09 AM   #1
Junior Member
Location: barcelona

Join Date: Sep 2018
Posts: 1
Default Create CSS with different adapters


I've searched for a similar thread and wasn't able to find a solution so I hope that this question is not repeated.

I am sequencing some SMRT library that contains the PacBio SMRT sequencing adapter in one end of the reads and a different adapter in the other.
I would like to create a CCS from these and I haven't been able to find any software that helps me doing that.

I have been looking to SMRT Link, which works only with SMRT adapters. Is there a way to introduce a custom adapter sequence?
I have also tried BBMap but I could only trim the adapters that are on the ends of the reads, not on the middle of them in order to create the CCS.
I was thinking on generating a custom script to do that but I find some difficulties like the mutation of sequencing, etc.

Can someone help me to find a useful software for my problem?

Thank you very much!!
JMir is offline   Reply With Quote

adapter remove, ccs, smrt

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:18 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO