Hi,
I'm trying to make a library of RNA following the ScriptSeq protocol. Everything works just fine except the RiboZero depletion of rRNA. I'm using young leafs of rice. An input of 2.5 µg of sample, 8 µl of removal solution.
I have to do this step twice and even then, I don't get rid of all the rRNA. I still have some nice peaks on the bioanalyzer.
I'm trying to make a library of RNA following the ScriptSeq protocol. Everything works just fine except the RiboZero depletion of rRNA. I'm using young leafs of rice. An input of 2.5 µg of sample, 8 µl of removal solution.
I have to do this step twice and even then, I don't get rid of all the rRNA. I still have some nice peaks on the bioanalyzer.
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