Hi all, when ligating adaptors and indexes, I have tried to amplify multiplexes from the 1st round of PCR in MASTR Dx BRCA assay using Q5 polymerase (ie, I left out completely Taq polymerase and something Multiplicom calles "universal PCR mix"). the products are longer (in yellow, please see the link), my only question is, should I be worried about the short bands around 120bp (presumably some nasty dimers)? Have you ever seen it in your reactions? thanx in advance for any suggestions..
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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