I was hoping to get some help regarding this issue –
I’m currently running the Truseq stranded mRNA library prep protocol with the nextseq for the purpose of generating a RNAseq library of cell lines.
What I’ve been seeing with my last several runs was a 3’ bias for certain genes, but not for others. In addition to that I’m also seeing muted expression profiles across the gene and uneven expression across the exons.
I’m currently running the Truseq stranded mRNA library prep protocol with the nextseq for the purpose of generating a RNAseq library of cell lines.
What I’ve been seeing with my last several runs was a 3’ bias for certain genes, but not for others. In addition to that I’m also seeing muted expression profiles across the gene and uneven expression across the exons.