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Old 04-20-2011, 03:06 AM   #1
hbn
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Default Experience with Illumina TruSeq kit for NGS

Hello everyone,

I am considering purchasing Illumina's TruSeq kit for multiplex sequencing. Does anyone have any experience with this? I am wandering if its very complicated and if it takes a lot of time to get experienced with it. Is it difficult to get good results with it? How many samples does it approx. take to get 'the hang of it'?

Can anyone give advice on this?
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Old 04-20-2011, 12:36 PM   #2
NextGenSeq
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I prefer the NuGEN Encore multiplex kit. The protocol is easy and gives good libraries. It only takes 3 hours to do too.
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Old 04-20-2011, 01:04 PM   #3
HESmith
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The TruSeq kit works well (i.e., produces diverse libraries, gives high quality sequence reads, demultiplexes without significant sample loss), but takes longer than the NuGEN kit. There are a number of manipulations but, if you have experience with molecular biology, the protocol is not difficult.
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Old 05-19-2011, 06:41 PM   #4
ScottC
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The TruSeq kit is also a LOT cheaper... at least, it is with our Illumina pricing in Australia (YMMV).

Cheers,

Scott.
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Old 05-19-2011, 07:27 PM   #5
monad
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We've made more than 1200 library since early this year using TruSeq. Very robust and good quality for Illumina sequencing. Much simplified compared to prior version of library kit.
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