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Old 05-11-2017, 02:28 PM   #1
Junior Member
Location: Chicago

Join Date: Mar 2017
Posts: 6
Default UMI in Smart-seq2 protocol

Hi All,

Has anyone had any experience trying to add UMI to the smart-seq2 protocol? I've done some searching, but I haven't found an account of anyone doing so. I would like to know if it is even possible.

Thank you!
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Old 05-11-2017, 04:10 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238

For this protocol UMI can be added to RT primer and SMART oligo. But they will be lost once amplified cDNA is fragmented/tagmented. With some modifications it is possible to keep primer and SMART oligo UMIs but reads from main body of transcript (except termini) will not have UMI.
nucacidhunter is offline   Reply With Quote

cdna synthesis, rna-seq, smart-seq2, umi

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