MiSeq

PGT_Bordeaux · 04-21-2011, 08:47 AM

Hello everybody,

I'm new, I work in facility of Bordeaux (France).
I have a GAIIx since November 2010, on this machine all is ok.

I saw the new sequencer illumina, the MySeq,
What some people are using it ?

manip time for make library ? on paper, time preparation is very fast ...
Multiplexing on 96 samples ??

krobison · 04-24-2011, 03:07 PM

MiSeq will not be available in quantity until the end of 2011; none have been officially sold yet (anyone out there alpha testing one?)

DNA Sorcerer · 07-25-2011, 01:26 PM

I though that library prep is done the same way as for GAIIx.
The main differences are in throughput. Myseq has only one "lane" and gives about 1GB when doing 150bp PE in abut 27Hs. Of course myseq is way smaller and all the modules are built in.

ShaunMahony · 07-26-2011, 08:31 PM

Keith -- did you ever find anyone who is testing a MiSeq? Especially in the Boston area? I'm interested in talking to someone with experience on the platform.

krobison · 07-27-2011, 08:00 AM

Shaun: No, I haven't seen one in the flesh yet. I'd also love to hear first hand knowledge of it.

There is an Illumina user's meeting in Cambridge MA this September 8th; hard to imagine that MiSeq won't figure prominently.

ShaunMahony · 07-27-2011, 08:05 AM

Thanks for the heads-up about the Illumina meeting!

penpen · 07-29-2011, 08:36 PM

Quote:

Originally Posted by krobison

MiSeq will not be available in quantity until the end of 2011; none have been officially sold yet (anyone out there alpha testing one?)

hi krobison, do u know the firm shipping date of miseq. illumina rep promise us to give one at the end of june, but it seems delayed...

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04-21-2011, 08:47 AM	#1
PGT_Bordeaux Junior Member Location: Bordeaux France Join Date: Dec 2009 Posts: 3	MiSeq Hello everybody, I'm new, I work in facility of Bordeaux (France). I have a GAIIx since November 2010, on this machine all is ok. I saw the new sequencer illumina, the MySeq, What some people are using it ? manip time for make library ? on paper, time preparation is very fast ... Multiplexing on 96 samples ??

07-25-2011, 01:26 PM	#3
DNA Sorcerer Member Location: Canada Join Date: Mar 2010 Posts: 24	I though that library prep is done the same way as for GAIIx. The main differences are in throughput. Myseq has only one "lane" and gives about 1GB when doing 150bp PE in abut 27Hs. Of course myseq is way smaller and all the modules are built in. __________________ Hi there

04-24-2011, 03:07 PM	#2
krobison Senior Member Location: Boston area Join Date: Nov 2007 Posts: 747	MiSeq will not be available in quantity until the end of 2011; none have been officially sold yet (anyone out there alpha testing one?)

07-26-2011, 08:31 PM	#4
ShaunMahony Member Location: University Park, PA Join Date: Apr 2008 Posts: 27	Keith -- did you ever find anyone who is testing a MiSeq? Especially in the Boston area? I'm interested in talking to someone with experience on the platform.

07-27-2011, 08:00 AM	#5
krobison Senior Member Location: Boston area Join Date: Nov 2007 Posts: 747	Shaun: No, I haven't seen one in the flesh yet. I'd also love to hear first hand knowledge of it. There is an Illumina user's meeting in Cambridge MA this September 8th; hard to imagine that MiSeq won't figure prominently.

07-27-2011, 08:05 AM	#6
ShaunMahony Member Location: University Park, PA Join Date: Apr 2008 Posts: 27	Thanks for the heads-up about the Illumina meeting!