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06-16-2011, 03:42 AM
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Member
Location: France Join Date: May 2011
Posts: 25
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heterogeneity of results
We are working on sequencing human exome using agilent for enrichment and GAIIx for sequencing.
We got a high heterogeneity of read profiles from a DNA sample to an other. For example : for a given mutation, we got for 1 DNA sample a deph of 50X, for an other it will be 2X and so on. Is it a problem due to Agilent or Illumina? Did anybody read a paper about this issue? |
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